Identification of novel adenovirus genotype 90 in children from Bangladesh

Charlotte J. Houldcroft; Mathew A. Beale; Md Abu Sayeed; Firdausi Qadri; Gordon Dougan; Ankur Mutreja

doi:10.1099/mgen.0.000221

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oa Identification of novel adenovirus genotype 90 in children from Bangladesh

Authors: Charlotte J. Houldcroft^1,* , Mathew A. Beale² , Md Abu Sayeed³ , Firdausi Qadri³ , Gordon Dougan¹ , Ankur Mutreja¹
- VIEW AFFILIATIONS
- ¹ 1Department of Medicine, University of Cambridge, Cambridge, UK ² 2Wellcome Sanger Institute, Hinxton, Cambridgeshire, UK ³ 3International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh
*Correspondence: Charlotte J. Houldcroft [email protected]
First Published Online: 24 September 2018, Microbial Genomics , 2018 4, doi: 10.1099/mgen.0.000221
Subject: Short Paper - Microbial Evolution and Epidemiology - Communicable Disease Genomics

Received: 12/07/2018
Accepted: 29/08/2018
Cover date: 01/10/2018

This is an open access article published by the Microbiology Society under the Creative Commons Attribution License

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Novel adenovirus genotypes are associated with outbreaks of disease, such as acute gastroenteritis, renal disease, upper respiratory tract infection and keratoconjunctivitis. Here, we identify novel and variant adenovirus genotypes in children coinfected with enterotoxigenic Escherichia coli, in Bangladesh. Metagenomic sequencing of stool was performed and whole adenovirus genomes were extracted. A novel species D virus, designated genotype 90 (P33H27F67) was identified, and the partial genome of a putative recombinant species B virus was recovered. Furthermore, the enteric types HAdV-A61 and HAdV-A40 were found in stool specimens. Knowledge of the diversity of adenovirus genomes circulating worldwide, especially in low-income countries where the burden of disease is high, will be required to ensure that future vaccination strategies cover the diversity of adenovirus strains associated with disease.

All supporting data, code and protocols have been provided within the article or through supplementary data files. Five supplementary figures are available with the online version of this article.

Keyword(s): whole-genome sequencing, metagenomics, diarrhoeal disease, de novo assembly, DNA virus, adenovirus

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